Departmental Profile

College of Veterinary Science, Khanapara  ::  Animal Biotechnology

Name of the Head of the Department:   Dr. Probodh Borah

Address:   Department of Animal Biotechnology College of Veterinary Science, AAU, Khanapara, Guwahati - 781022

Phone:   9435116191

Contact email:   borahp@vetbifguwahati.ernet.in

Number of Staff in Department:   8         Staff Details        View Profiles

Total Intake Capacity:   10         Details of Course Offered

About the Salient Achievements of the Department:    Molecular fingerprinting of six different species of domestic animals of north-eastern region, namely cattle, buffalo, pig, yak, sheep and goat was done by PCR-RFLP based on 12S rRNA and 16S rRNA genes of mitochondrial DNA. A multiplex PCR protocol was developed for rapid detection of pathogroups of E. coli isolates from man, animals and birds based on their virulence gene profile. A rapid, high throughput multiplex PCR assay based on 16 genes variably distributed in various Salmonella serovars was developed for identification of common clinical serovars of Salmonella within 2-3 hours. Virulence and antimicrobial-resistance gene profiles of Salmonella isolates from animals and man have been studied. A globally accessible online server has been developed on “Serotyping of common clinical isolates of Salmonella by Multiplex PCR” in collaboration with Vaccine Research Institute of San Diego, California (URL: http://182.71.179.101/smart/index.php). Reverse-transcriptase PCR (RT-PCR) method was standardized for detection of FMD virus serotype ‘O’ in clinical samples and its comparative efficacy was evaluated compared to sandwich ELISA and virus isolation. RT-PCR protocol for detection of Toll-Like Receptor-3 (TLR-3) in FMDV-infected tissue was also standardized. A study was also conducted on persistence of FMD virus type ‘O’ in cattle in Assam. Incidence of polymorphism in fecundity related genes, GDF9 and BMPR1B in Assam Hill goat was studied by PCR-RFLP. The study revealed that the genetic factor responsible for prolificacy or multiple kidding rates is not related to the reported mutated alleles at GDF9 and BMPR1B major genes. Relative expression of genes coding for the enzymes, Fatty Acid Synthase (FAS) and Stearoyl CoA Desaturase (SCD) and three major transcription factors, Sterol Regulatory Element Binding Protein (SREBP), Peroxisome Proliferator Activated Receptor γ (PPAR γ) and CCAAT-enhancer-binding proteins (CEBP) related to lipogenic processes were analyzed by real-time PCR to establish zinc-mediated hormonal regulation (Growth Hormone, Insulin and Leptin) of fatty acid synthesis and desaturation in adipose tissue. Molecular characterization of SLC11A1 gene in doom pigs of Assam has been carried out and polymorphism has been detected. A study has also been made to assess DNA integrity and in-vivo sperm fertilizing capacity of frozen semen of bulls. Molecular characterization of β-lactoglobulin (β-Lg) and kappa-casein (κ-Cn) Genes in indigenous cattle of Assam has been done and polymorphism has been detected in both the genes. Under the DBT-funded BIF, an online database on Indigenous Livestock and Poultry Resources of North East India (ILRONE) has been developed. The database is accessible in the Internet through public IP: http://182.71.179.101/ilrone/project.

Details of Externally Funded Research/Infrastructure Projects:

Name Funding Agency Project Duration Total Fund Received Status

Molecular Tying and Virulence Characteristics of Salmonella with Special Reference to Non-host-specific Serovars DBT, Govt. of India 3 Years Rs. 79.05 lakhs Completed
Creation of an Online Repository of Biotechnology and Bioinformatics Resources of North-East India (BABRONE) DBT, Govt. of India 3 Years Rs. 28.91 lakhs Ongoing
Analysis of Gut Metagenome of Duck (Anas platyrhynchos) with special reference to Identification of Bacteria having Probiotic Potential DBT, Govt. of India 3 years Rs. 42.48 lakhs Ongoing
Study on persistence of Japanese Encephalitis in reservoir host, pig in JE endemic area of Odisha, Manipur and Assam IBSD, Imphal 2 years Rs. 26.73 lakhs Completed
Generation of DNA vaccine with a mutated capsid gene of porcine circovirus type 2 and evaluation of its immuno-potential NECBH-Twinning Program, DBT, Govt. of India 2 years Rs. 4.887 lakhs Ongoing
In-silico characterization and biological validation of potential peptide vaccines for Salmonella Typhi using the outer membrane protein PagN as target NECBH-Twinning Program, DBT, Govt. of India 2 years Rs. 2.99 lakhs Ongoing
Modelling of indigenous diagnostics and immuno-potent vaccine candidates to combat African swine fever in India DBT, Govt. of India 3 years Rs.22 Lakh Ongoing
Generation of multivalent vectored vaccine against Lumpy Skin Disease in cattle and evaluation of its immunogenic potency Assam Science, Technology & Environment Council 2 years Rs.8 Lakh Ongoing
Bioinformatics Infrastructure Facility (BIF) DBT, Govt. of India 14 years 67.81 lakhs Completed
Advanced Level State Biotech Hub (Phase-I) DBT, Govt. of India 9 years 343.7 lakhs Completed
M.V.Sc. programme in Animal Biotechnology DBT, Govt. of India 15 years 93.44 lakhs + Ongoing
Development of a recombinant subunit vaccine against swine pasteurellosis and evaluation of its immune protective efficacy Assam Science, Technology & Environment Council 2 years 2.05 lakhs Ongoing
Development of a consortium of bacteriophage for phage therapy of necrotic enteritis in poultry Ecophage Biotech, Bangalore 2 years 7.00 lakhs Ongoing
‘Promoting advance research and capacity building in biotechnology & allied disciplines through manpower training, mentoring and sharing of infrastructure developed under the Advanced-Level State Biotech Hub’ DBT, Govt. of India 3 years Rs. 4.32 crores Ongoing
Antibody engineering to develop rapid and cost-effective diagnostics for detection of Canine Distemper Virus (CDV) and Canine Parvovirus (CPV) infections Assam Agricultural University, Jorhat 3 years Rs. 25.00 lakh Ongoing
Development of a marketable probiotic consortium for poultry Assam Agricultural University 2 years 15 lacs Ongoing
Development of a cost-effective peptide-based indirect ELISA for sero-monitoring of African Swine Fever Assam Agricultural University 2 years 15 lacs Ongoing
Development and evaluation of multiple antigenic peptide-based diagnostic assay for detection of Babesia gibsoni in dogs targeting GPI-encoded protein Assam Science Technology and Environment Council 2 years 4.6 lacs Ongoing

Other Activities (e.g., National/International Conferences, Symposia, Seminar, etc. organized during the last 10 years):

Staff Details

Number of Professor :   1
Number of Associate Professor :   0
Number of Assistant Professor :   6
Number of Lecturer :   0
Number of other teaching staff :   0Number of Professor :   1
Number of Associate Professor :   1
Number of Assistant Professor :   6
Number of Lecturer :   0
Number of other teaching staff :   0Number of Professor :   1
Number of Associate Professor :   1
Number of Assistant Professor :   6
Number of Lecturer :   0
Number of other teaching staff :   0
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Course Details

Course Name

Duration

Intake Capacity

MVSc 2 Years 10
PhD 3 Years 2

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Name	Funding Agency	Project Duration	Total Fund Received	Status
Molecular Tying and Virulence Characteristics of Salmonella with Special Reference to Non-host-specific Serovars	DBT, Govt. of India	3 Years	Rs. 79.05 lakhs	Completed
Creation of an Online Repository of Biotechnology and Bioinformatics Resources of North-East India (BABRONE)	DBT, Govt. of India	3 Years	Rs. 28.91 lakhs	Ongoing
Analysis of Gut Metagenome of Duck (Anas platyrhynchos) with special reference to Identification of Bacteria having Probiotic Potential	DBT, Govt. of India	3 years	Rs. 42.48 lakhs	Ongoing
Study on persistence of Japanese Encephalitis in reservoir host, pig in JE endemic area of Odisha, Manipur and Assam	IBSD, Imphal	2 years	Rs. 26.73 lakhs	Completed
Generation of DNA vaccine with a mutated capsid gene of porcine circovirus type 2 and evaluation of its immuno-potential	NECBH-Twinning Program, DBT, Govt. of India	2 years	Rs. 4.887 lakhs	Ongoing
In-silico characterization and biological validation of potential peptide vaccines for Salmonella Typhi using the outer membrane protein PagN as target	NECBH-Twinning Program, DBT, Govt. of India	2 years	Rs. 2.99 lakhs	Ongoing
Modelling of indigenous diagnostics and immuno-potent vaccine candidates to combat African swine fever in India	DBT, Govt. of India	3 years	Rs.22 Lakh	Ongoing
Generation of multivalent vectored vaccine against Lumpy Skin Disease in cattle and evaluation of its immunogenic potency	Assam Science, Technology & Environment Council	2 years	Rs.8 Lakh	Ongoing
Bioinformatics Infrastructure Facility (BIF)	DBT, Govt. of India	14 years	67.81 lakhs	Completed
Advanced Level State Biotech Hub (Phase-I)	DBT, Govt. of India	9 years	343.7 lakhs	Completed
M.V.Sc. programme in Animal Biotechnology	DBT, Govt. of India	15 years	93.44 lakhs +	Ongoing
Development of a recombinant subunit vaccine against swine pasteurellosis and evaluation of its immune protective efficacy	Assam Science, Technology & Environment Council	2 years	2.05 lakhs	Ongoing
Development of a consortium of bacteriophage for phage therapy of necrotic enteritis in poultry	Ecophage Biotech, Bangalore	2 years	7.00 lakhs	Ongoing
‘Promoting advance research and capacity building in biotechnology & allied disciplines through manpower training, mentoring and sharing of infrastructure developed under the Advanced-Level State Biotech Hub’	DBT, Govt. of India	3 years	Rs. 4.32 crores	Ongoing
Antibody engineering to develop rapid and cost-effective diagnostics for detection of Canine Distemper Virus (CDV) and Canine Parvovirus (CPV) infections	Assam Agricultural University, Jorhat	3 years	Rs. 25.00 lakh	Ongoing
Development of a marketable probiotic consortium for poultry	Assam Agricultural University	2 years	15 lacs	Ongoing
Development of a cost-effective peptide-based indirect ELISA for sero-monitoring of African Swine Fever	Assam Agricultural University	2 years	15 lacs	Ongoing
Development and evaluation of multiple antigenic peptide-based diagnostic assay for detection of Babesia gibsoni in dogs targeting GPI-encoded protein	Assam Science Technology and Environment Council	2 years	4.6 lacs	Ongoing